Principles of light microscope
Various parts of microscope
Uses of microscope
Cleaning and maintenance of microscope
Various attachments of compound
microscope (principle only)

Principle, significance and
interpretation of PAS (Periodic Acid
Schiff’s Reagent) AFB and Leprae

Demonstration of various parts of
Optical) light microscope (Mechanical
& and cryostat

Principle, significance and interpretation
of Masson’s trichrome stain,Oil Red
O and Gram’s stain

decalcification

Reception and processing of frozen tissue

Processing of tissue for
frozen section

Freezing microtome and cryostat,
Advantages and dis-advantages of
freezing microtome

Staining and mounting of frozen
section using H&E stain